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5.08J Biological Chemistry II (Spring 2016, MIT OCW). Instructors: Prof. JoAnne Stubbe and Prof. Elizabeth Nolan.
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English [CC]
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Description
This course is an advanced treatment of biochemical mechanisms that underlie biological processes. Topics include macromolecular machines such as the ribosome, the proteasome, fatty acid synthases as a paradigm for polyketide synthases and nonribosomal polypeptide synthases, and polymerases. Emphasis will be given to the experimental methods used to unravel how these processes fit into the cellular context as well as the coordinated regulation of these processes. (from ocw.mit.edu)
Course content
- Lecture 01 – Introduction Unlimited
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- Lecture 02 – Protein Synthesis 1 Unlimited
- Recitation 01 – Determining, Analyzing, and Understanding Protein Structures Unlimited
- Lecture 03 – Protein Synthesis 2 Unlimited
- Lecture 04 – Protein Synthesis 3 Unlimited
- Lecture 05 – Protein Synthesis 4 Unlimited
- Recitation 02 – Pre-steady State and Steady-state Kinetic Methods Applied to Translation Unlimited
- Lecture 06 – Protein Synthesis 5 Unlimited
- Lecture 07 – Protein Synthesis 6 Unlimited
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- Lecture 08 – Protein Folding 1 Unlimited
- Recitation 03 – Pre-steady State and Steady-state Kinetic Methods Applied to Translation Unlimited
- Lecture 09 – Protein Folding 2 Unlimited
- Lecture 10 – Protein Folding 3 Unlimited
- Lecture 11 – Protein Folding 4 Unlimited
- Recitation 04 – Purification of Native and Mutant Ribosomes, Protein Purification Unlimited
- Lecture 12 – Protein Degradation 1 Unlimited
- Lecture 13 – Protein Degradation 2 Unlimited
- Lecture 14 – Protein Degradation 3 Unlimited
- Recitation 05 – Overview of Cross-Linking, Including Photoreactive Cross-Linking Methods Unlimited
- Recitation 07 – Application of Single Molecule Methods Unlimited
- Lecture 19 – Cholesterol Biosynthesis 1 Unlimited
- Lecture 20 – Cholesterol Biosynthesis 2 Unlimited
- Lecture 21 – Cholesterol Biosynthesis 3 and Cholesterol Homeostasis 1 Unlimited
- Recitation 08 – Application of CRISPR to Study Cholesterol Regulation Unlimited
- Lecture 22 – Cholesterol Homeostasis 2 Unlimited
- Lecture 23 – Cholesterol Homeostasis 3 Unlimited
- Lecture 24 – Cholesterol Homeostasis 4 Unlimited
- Recitation 09 – Cholesterol Homeostasis and Sensing Unlimited
- Lecture 25 – Cholesterol Homeostasis 5 and Metal Ion Homeostasis 1 Unlimited
- Recitation 12 – Mass Spectrometry of the Cysteine Proteome Unlimited
- Lecture 32 – Reactive Oxygen Species 2 Unlimited
- Lecture 33 – Reactive Oxygen Species 3 Unlimited
- Lecture 34 – Reactive Oxygen Species 4 and Nucleotide Metabolism 1 Unlimited
Instructor
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