0

(

ratings

)

1

students

Created by:

Profile Photo

Last updated:

September 25, 2023

Duration:

Unlimited Duration

FREE

This course includes:

Unlimited Duration

Badge on Completion

Certificate of completion

Unlimited Duration

Description

Analytical Technologies in Biotechnology. Instructor: Dr. Ashwani K. Sharma, Department of Biotechnology, IIT Roorkee.

Welcome to this course on analytical technologies in biotechnologies. It is quite evident that advances in technology has been vital in the progress of science. In life sciences, advances in analytical techniques have played a crucial role in understanding of biological functions at the molecular and the cellular label. Biotechnology is an interdisciplinary science that involves applications of chemical and physical techniques, in the field of life sciences. This course covers lessons on Microscopy, Radioisotope techniques, Chromatographic methods, Electrophoresis, Centrifugation techniques, Spectroscopic techniques, Polymerase Chain Reaction(PCR), DNA sequencing, and Enzyme linked immunosorbent assay. (from nptel.ac.in)

Course Curriculum

    • Lecture 01 – Basic Concepts in Microscopy Unlimited
    • Lecture 02 – Basic Concepts in Microscopy (cont.) Unlimited
    • Lecture 03 – Dark-field and Phase Contrast Microscopy Unlimited
    • Lecture 04 – Differential Interference Contrast and Polarization Microscopy Unlimited
    • Lecture 05 – Fluorescence and Confocal Microscopy Unlimited
    • Lecture 06 – Transmission Electron Microscopy Unlimited
    • Lecture 07 – Transmission Electron Microscopy (cont.), Scanning Electron Microscopy Unlimited
    • Lecture 08 – Basic Concepts Unlimited
    • Lecture 09 – Basic Concepts (cont.) Unlimited
    • Lecture 10 – GM Counting and Scintillation Counting Unlimited
    • Lecture 11 – Scintillation Counting (cont.) Unlimited
    • Lecture 12 – Autoradiography and RIA Unlimited
    • Lecture 13 – Safety Aspects and Applications Unlimited
    • Lecture 14 – Introduction and Basic Concepts in Chromatography Unlimited
    • Lecture 15 – Basic Concepts in Chromatography Unlimited
    • Lecture 16 – Low-Pressure Liquid Chromatography (LPLC) and High Performance … Unlimited
    • Lecture 17 – Ion-exchange Chromatography Unlimited
    • Lecture 18 – Gel Filtration Chromatography Unlimited
    • Lecture 19 – Affinity Chromatography Unlimited
    • Lecture 20 – Gas-Liquid Chromatography Unlimited
    • Lecture 21 – Basis Concept in Electrophoresis Unlimited
    • Lecture 22 – Horizontal and Vertical Gel Electrophoresis Unlimited
    • Lecture 23 – Native Gel Electrophoresis and SDS-PAGE Unlimited
    • Lecture 24 – Isoelectric Focusing, 2D Gel Electrophoresis and Protein Detection Methods Unlimited
    • Lecture 25 – Electrophoresis of Nucleic Acids Unlimited
    • Lecture 26 – Immunoelectrophoresis and Capillary Electrophoresis Unlimited
    • Lecture 27 – Introduction and Basic Concepts Unlimited
    • Lecture 28 – Basic Concepts (cont.) Unlimited
    • Lecture 29 – Types of Centrifuges and Analytical Ultracentrifugation Method Unlimited
    • Lecture 30 – Separation Methods in Preparative Ultracentrifuges Unlimited
    • Lecture 31 – Types of Rotors Unlimited
    • Lecture 32 – Types of Rotors (cont.), Care of Rotors Unlimited
    • Lecture 33 – Introduction and Basic Concepts Unlimited
    • Lecture 34 – UV-Visible Spectroscopy Unlimited
    • Lecture 35 – Infrared and Fluorescence Spectroscopy Unlimited
    • Lecture 36 – Circular Dichroism (CD) Spectroscopy Unlimited
    • Lecture 37 – Nuclear Magnetic Resonance (NMR) Spectroscopy and X-ray Crystallography Unlimited
    • Lecture 38 – Atomic Spectroscopy and Mass Spectrometry Unlimited
    • Lecture 39 – Polymerase Chain Reaction (PCR) Unlimited
    • Lecture 40 – DNA Sequencing Methods Unlimited
    • Lecture 41 – Enzyme Linked Immunosorbent Assay (ELISA) Unlimited

About the instructor

5 5

Instructor Rating

6

Reviews

4637

Courses

24151

Students

Profile Photo
OpenCoursa
We are an educational and skills marketplace to accommodate the needs of skills enhancement and free equal education across the globe to the millions. We are bringing courses and trainings every single day for our users. We welcome everyone woth all ages, all background to learn. There is so much available to learn and deliver to the people.