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Analytical Technologies in Biotechnology. Instructor: Dr. Ashwani K. Sharma, Department of Biotechnology, IIT Roorkee.
1138 years, 9 months
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Welcome to this course on analytical technologies in biotechnologies. It is quite evident that advances in technology has been vital in the progress of science. In life sciences, advances in analytical techniques have played a crucial role in understanding of biological functions at the molecular and the cellular label. Biotechnology is an interdisciplinary science that involves applications of chemical and physical techniques, in the field of life sciences. This course covers lessons on Microscopy, Radioisotope techniques, Chromatographic methods, Electrophoresis, Centrifugation techniques, Spectroscopic techniques, Polymerase Chain Reaction(PCR), DNA sequencing, and Enzyme linked immunosorbent assay. (from nptel.ac.in)
Course Currilcum
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- Lecture 01 – Basic Concepts in Microscopy Unlimited
- Lecture 02 – Basic Concepts in Microscopy (cont.) Unlimited
- Lecture 03 – Dark-field and Phase Contrast Microscopy Unlimited
- Lecture 04 – Differential Interference Contrast and Polarization Microscopy Unlimited
- Lecture 05 – Fluorescence and Confocal Microscopy Unlimited
- Lecture 06 – Transmission Electron Microscopy Unlimited
- Lecture 07 – Transmission Electron Microscopy (cont.), Scanning Electron Microscopy Unlimited
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- Lecture 08 – Basic Concepts Unlimited
- Lecture 09 – Basic Concepts (cont.) Unlimited
- Lecture 10 – GM Counting and Scintillation Counting Unlimited
- Lecture 11 – Scintillation Counting (cont.) Unlimited
- Lecture 12 – Autoradiography and RIA Unlimited
- Lecture 13 – Safety Aspects and Applications Unlimited
- Lecture 14 – Introduction and Basic Concepts in Chromatography Unlimited
- Lecture 15 – Basic Concepts in Chromatography Unlimited
- Lecture 16 – Low-Pressure Liquid Chromatography (LPLC) and High Performance … Unlimited
- Lecture 17 – Ion-exchange Chromatography Unlimited
- Lecture 18 – Gel Filtration Chromatography Unlimited
- Lecture 19 – Affinity Chromatography Unlimited
- Lecture 20 – Gas-Liquid Chromatography Unlimited
- Lecture 27 – Introduction and Basic Concepts Unlimited
- Lecture 28 – Basic Concepts (cont.) Unlimited
- Lecture 29 – Types of Centrifuges and Analytical Ultracentrifugation Method Unlimited
- Lecture 30 – Separation Methods in Preparative Ultracentrifuges Unlimited
- Lecture 31 – Types of Rotors Unlimited
- Lecture 32 – Types of Rotors (cont.), Care of Rotors Unlimited
- Lecture 39 – Polymerase Chain Reaction (PCR) Unlimited
- Lecture 40 – DNA Sequencing Methods Unlimited
- Lecture 41 – Enzyme Linked Immunosorbent Assay (ELISA) Unlimited
