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December 5, 2022


Unlimited Duration


This course includes:

Unlimited Duration

Badge on Completion

Certificate of completion

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Directed evolution has been used to produce enzymes with many unique properties.

The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications.

This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. Many instructors of the Advanced Undergraduate Seminars are postdoctoral scientists with a strong interest in teaching.

Course Curriculum

  • Introduction Unlimited
  • Library generation by point mutation Unlimited
  • Library generation by recombination Unlimited
  • Alternative methods for library generation Unlimited
  • Enzyme evolution by genetic complementation Unlimited
  • Enzyme evolution by chemical complementation Unlimited
  • Enzyme evolution using phage display (cont.) Unlimited
  • Enzyme evolution using bacterial cell surface display Unlimited
  • Enzyme evolution using yeast surface display Unlimited
  • Enzyme evolution using ribosome display Unlimited
  • Enzyme evolution by in vitro compartmentalization Unlimited
  • Alternative methods for enzyme/catalyst design Unlimited

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Massachusetts Institute of Technology