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Last updated:

December 13, 2022

Duration:

Unlimited Duration

FREE

This course includes:

Unlimited Duration

Badge on Completion

Certificate of completion

Unlimited Duration

Description

he course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec.

echniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing.

This class is part of the new laboratory curriculum in the MIT Department of Chemistry. Undergraduate Research-Inspired Experimental Chemistry Alternatives (URIECA) introduces students to cutting edge research topics in a modular format.

Acknowledgments

Development of this course was funded through an HHMI Professors grant to Professor Catherine L. Drennan.

Course Curriculum

  • Introduction. Kinases in healthy and cancerous cells Unlimited
  • DNA digestion and PCR Unlimited
  • Affinity tags for protein purification/detection Unlimited
  • Conserved and variable structural features of kinase domains Unlimited
  • Site directed mutagenesis (Quikchange method) Unlimited
  • Detecting kinase activity Unlimited

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Massachusetts Institute of Technology
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