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Description
he course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec.
echniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing.
This class is part of the new laboratory curriculum in the MIT Department of Chemistry. Undergraduate Research-Inspired Experimental Chemistry Alternatives (URIECA) introduces students to cutting edge research topics in a modular format.
Acknowledgments
Development of this course was funded through an HHMI Professors grant to Professor Catherine L. Drennan.
Course Curriculum
- Introduction. Kinases in healthy and cancerous cells Unlimited
- DNA digestion and PCR Unlimited
- Affinity tags for protein purification/detection Unlimited
- Conserved and variable structural features of kinase domains Unlimited
- Site directed mutagenesis (Quikchange method) Unlimited
- Detecting kinase activity Unlimited
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