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Last updated:

September 25, 2023

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Description

Interactomics: Protein Arrays and Label-free Biosensors. Instructor: Prof. Sanjeeva Srivastava, Department of Biosciences and Bioengineering, IIT Bombay.

Proteins are the key effectors of any living system and are largely responsible for the functioning of a cell. Intricate cell signaling and molecular triggers are dependent on interactions involving proteins at the cellular level. It is due to this very reason that, in an age where clinical biology is thriving to make an impact in global health-care and biomedical diagnostics, there has been a surge of interest in the area of Interactomics. Interactomics essentially involves the study of interactions between biomolecules, particularly proteins and the consequences of those interactions in a biosystem.

This course of interactomics focuses on two powerful platforms: 1. Protein microarrays and 2. Label free biosensors involving Surface Plasmon Resonance (SPR) and Biolayer Interferometry (BLI). While the former is a high-throughput tool for discovery-based interactomics, the later is proving itself to be an instrumental resource for validation based studies. This course details the overlapping domains of proteomics and interactomics, which form the focus of researchers making an impact in the clinical biomarker and pharmaceutical arena across the globe. (from nptel.ac.in)

Course Curriculum

  • Lecture 01 – Introduction to Interactomics Unlimited
  • Lecture 02 – An Overview of Label-Free Technologies Unlimited
  • Lecture 03 – An Overview of Surface Plasmon Resonance (SPR) Unlimited
  • Lecture 04 – An Overview of Surface Plasmon Resonance Imaging (SPRi) Unlimited
  • Lecture 05 – Basics of SPR: Surface Chemistry Unlimited
  • Lecture 06 – Basics of SPR: Experimental Design Unlimited
  • Lecture 07 – Protein Immobilization for Protein-Protein Interaction Studies Unlimited
  • Lecture 08 – Protein-Protein Interaction Study: Binding Analysis Unlimited
  • Lecture 09 – Protein-Protein Interaction Study: Kinetic Analysis Unlimited
  • Lecture 10 – Protein-Small Molecule Interaction Study: Immobilization and Binding Analysis Unlimited
  • Lecture 11 – Protein-Small Molecule Interaction Study: Kinetic Analysis Unlimited
  • Lecture 12 – SPR: Interactive Session Unlimited
  • Lecture 13 – SPR: Interactive Session (cont.) Unlimited
  • Lecture 14 – An Overview of Ellipsometry and Interferometry Techniques Unlimited
  • Lecture 15 – An Introduction to Biolayer Interferometry and its Applications in Protein Research Unlimited
  • Lecture 16 – Kinetic Analysis of Protein-Protein Interaction Using Biolayer Interferometry Unlimited
  • Lecture 17 – Label-Free Quantification of Proteins Using Biolayer Interferometry Unlimited
  • Lecture 18 – Diffraction based Biosensors Unlimited
  • Lecture 19 – Diffraction based Biosensors (cont.) Unlimited
  • Lecture 20 – Nanotechniques in Proteomics Unlimited
  • Lecture 21 – Nanotechniques in Proteomics (cont.) Unlimited
  • Lecture 22 – High-throughput Platforms of Interactomics: Protein Arrays Unlimited
  • Lecture 23 – Conventional Label-based Detection Techniques for Protein Microarrays Unlimited
  • Lecture 24 – Novel Detection Techniques for Protein Microarrays Unlimited
  • Lecture 25 – Recombinational Cloning and its Application for Protein Microarrays Unlimited
  • Lecture 26 – An Introduction to Cell-free Protein Synthesis Unlimited
  • Lecture 27 – Cell-free Synthesis based Protein Microarrays PISA and NAPPA Unlimited
  • Lecture 28 – Cell-free Synthesis based Protein Microarrays MIST DAPA and Halotag Arrays Unlimited
  • Lecture 29 – Digging Deeper into NAPPA: Basic Workflow Unlimited
  • Lecture 30 – Digging Deeper into NAPPA: Basic Workflow Surface Chemistry Printing and Assessment Unlimited
  • Lecture 31 – Application of Cell-free Expression Protein Microarrays in Biomarker Discovery Unlimited
  • Lecture 32 – Application of Cell-free Expression Protein Microarrays in Immunological Studies Unlimited
  • Lecture 33 – Basics of Microarray Image Scanning Unlimited
  • Lecture 34 – Software for Image Scanning and Data Processing Unlimited
  • Lecture 35 – MicroArray Data Analysis Unlimited
  • Lecture 36 – MicroArray Data Analysis (cont.) Unlimited
  • Lecture 37 – Application of Protein Microarray in Biomarker Discovery Unlimited
  • Lecture 38 – Application of Protein Microarray in Biomarker Discovery (cont.) Unlimited
  • Lecture 39 – Systems Biology and Networks Unlimited
  • Lecture 40 – Challenges in Proteomics Unlimited

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