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Last updated:

April 8, 2022


Unlimited Duration

This course includes:

Unlimited Duration

Badge on Completion

Certificate of completion

Unlimited Duration


This course provides an introduction to microscopy and the operation of a simple light microscope, of the type found in histology courses and teaching laboratories. It outlines different methods used for preparing and staining tissue sections for microscopy and explains how different strains can be used to identify particular cells, pathogens and anatomical structures.

Course learning outcomes

After studying this course, you should be able to:
  • identify and understand the principle components of a light microscope
  • understand in outline, the principles of optics that apply to light microscopes
  • set up and use a light microscope
  • understand why different staining procedures are used on tissue sections
  • understand the principles of immunohistochemistry.

Course Curriculum

    • 1 Microscopy 00:10:00
    • 1.1 The light microscope 00:55:00
    • 1.2 Using a light microscope 00:40:00
    • 2.1 Phase contrast 00:40:00
    • 2.2 Filters 00:10:00
    • 2.3 Grids and graticules 00:20:00
    • 3.1 Histochemical techniques 00:30:00
    • 4.1 Immunohistochemistry (IHC) 00:30:00
    • 5.1 Interpreting the appearance of sections 00:20:00
    • 5.2 The Z-plane 00:10:00
    • 6.1 Fluorescence microscopes 00:20:00
    • 6.2 The fluorescence confocal microscope 00:20:00
    • Conclusion 00:10:00

About the instructor

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