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Last updated:

December 7, 2022

Duration:

Unlimited Duration

FREE

This course includes:

Unlimited Duration

Badge on Completion

Certificate of completion

Unlimited Duration

Description

This course introduces experimental biochemical and molecular techniques from a quantitative engineering perspective.

Experimental design, data analysis, and scientific communication form the underpinnings of this subject. Three discovery-based experimental modules focus on RNA engineering, protein engineering, and cell-biomaterial engineering.

This OCW site is based on the source OpenWetWare class Wiki, 20.109(S10): Laboratory Fundamentals of Biological Engineering.

Course Curriculum

  • Orientation Unlimited
    • Writing and communications introduction (Neal Lerner) Unlimited
    • The Language of Biological Engineering Reports (Linda Sutliff) Unlimited
    • Creating your 20.109 presentation (Atissa Banuazizi) Unlimited
    • Introduction Unlimited
    • Rational protein design Unlimited
    • Fluorescence and sensors Unlimited
    • Protein expression Unlimited
    • Review & gene analysis Unlimited
    • Purification and protein analysis Unlimited
    • Binding & affinity measurements Unlimited
    • High throughput engineering Unlimited
    • Introduction Unlimited
    • Introduction to biomaterials; cartilage composition Unlimited
    • Basic statistics; standards in scientific communities I Unlimited
    • Standards in scientific communities II; cell viability Unlimited
    • Assays for transcription and protein levels Unlimited
    • Cartilage TE: from in vitro and in vivo models to the clinic Unlimited
    • Creating your proposal presentation (Atissa Banuazizi) Unlimited

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Massachusetts Institute of Technology
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