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Last updated:

December 7, 2022

Duration:

Unlimited Duration

FREE

This course includes:

Unlimited Duration

Badge on Completion

Certificate of completion

Unlimited Duration

Description

This course introduces experimental biochemical and molecular techniques from a quantitative engineering perspective

Experimental design, rigorous data analysis, and scientific communication form the underpinnings of this subject. Three discovery-based experimental modules focus on genome engineering, expression engineering, and biomaterial engineering.

This OCW site is based on the source OpenWetWare class Wiki, found at 20.109(F07): Laboratory Fundamentals of Biological Engineering.

Course Curriculum

    • Start-up genome engineering Unlimited
    • Agarose gel electrophoresis Unlimited
    • DNA ligation and bacterial transformation Unlimited
    • Examine candidate clones Unlimited
    • M13.1 Unlimited
    • Lecture on oral presentations Unlimited
    • Western analysis Unlimited
    • Probe western Unlimited
    • siRNA design and introduction to cell culture Unlimited
    • Transfection Unlimited
    • Luciferase assays and RNA prep Unlimited
    • Lecture on written reports and scientific writing Unlimited
    • cDNA synthesis and microarray Unlimited
    • High throughput technologies Unlimited

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Massachusetts Institute of Technology
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