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Last updated:
December 7, 2022
Duration:
Unlimited Duration
FREE
This course includes:
Unlimited Duration
Badge on Completion
Certificate of completion
Unlimited Duration
Description
This course introduces experimental biochemical and molecular techniques from a quantitative engineering perspective
Experimental design, rigorous data analysis, and scientific communication form the underpinnings of this subject. Three discovery-based experimental modules focus on genome engineering, expression engineering, and biomaterial engineering.
This OCW site is based on the source OpenWetWare class Wiki, found at 20.109(F07): Laboratory Fundamentals of Biological Engineering.
Course Curriculum
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- Start-up genome engineering Unlimited
- Agarose gel electrophoresis Unlimited
- DNA ligation and bacterial transformation Unlimited
- Examine candidate clones Unlimited
- M13.1 Unlimited
- Lecture on oral presentations Unlimited
- Western analysis Unlimited
- Probe western Unlimited
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- siRNA design and introduction to cell culture Unlimited
- Transfection Unlimited
- Luciferase assays and RNA prep Unlimited
- Lecture on written reports and scientific writing Unlimited
- cDNA synthesis and microarray Unlimited
- High throughput technologies Unlimited
About the instructor
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Massachusetts Institute of Technology
