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September 25, 2023


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Proteins and Gel-Based Proteomics. Instructor: Prof. Sanjeeva Srivastava, Department of Biosciences and Bioengineering, IIT Bombay.

This course introduces to the basic biology of proteins and gel-based proteomics. To obtain better understanding of cellular processes and regulation, there has been an increasing interest in studying proteomics. Proteomics techniques aim to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. These proteomic techniques typically aim to elucidate the expression, interaction, and cellular function of proteins.

The course will provide the basic understanding of amino acids and proteins. It would also provide detailed information on gel-based proteomic techniques such as SDS-PAGE and 2-DE. Some of the limitation of 2-DE has been overcome by advanced Difference gel electrophoresis (DIGE) technique, which will be discussed in this course. Protein identification using MALDI-TOF/TOF mass spectrometry will also be discussed. 2-DE in combination with mass spectrometry has become very powerful tool for proteomics applications and students can learn these tools by attending this course. (from nptel.ac.in)

Course Curriculum

    • Lecture 01 – Course Introduction Unlimited
    • Lecture 02 – Introduction to Amino Acids Unlimited
    • Lecture 03 – Introduction to Proteins Unlimited
    • Lecture 04 – Protein Folding and Misfolding Unlimited
    • Lecture 05 – Protein Purification Techniques Unlimited
    • Lecture 06 – Introduction to Proteomics Unlimited
    • Lecture 07 – Systems Biology and Proteomics Unlimited
    • Lecture 08 – Sample Preparation and Pre-analytical Factors Unlimited
    • Lecture 09 – Sample Preparation: Pre-analytical Factors (cont.) Unlimited
    • Lecture 10 – Sample Preparation: Protein Extraction and Quantification Unlimited
    • Lecture 11 – One-dimensional Electrophoresis Unlimited
    • Lecture 12 – Rehydration, IEF and Equilibration Unlimited
    • Lecture 13 – Second Dimension, Staining and Destaining Unlimited
    • Lecture 14 – Gel Analysis Unlimited
    • Lecture 15 – Two-dimensional Gel Electrophoresis: Applications Unlimited
    • Lecture 16 – Two-dimensional Gel Electrophoresis: Applications and Challenges Unlimited
    • Lecture 17 – Two-dimensional DIGE: Basics Unlimited
    • Lecture 18 – Two-dimensional DIGE: Data Analysis Unlimited
    • Lecture 19 – Two-dimensional DIGE: Applications Unlimited
    • Lecture 20 – Protein Identification using MALDI-TOF/TOF Unlimited
    • Lecture 21 – Proteomics Experiment Data Analysis and Challenges Unlimited

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